Job Requisition Number: 23971. About the Heald Lab - Our interdisciplinary approaches take advantage of cytoplasmic extracts prepared from eggs of the frog Xenopus laevis that reconstitute mitotic chromosome condensation and spindle assembly and function in vitro. To study mechanisms of spindle and organelle size control, we utilize a smaller, related frog, Xenopus tropicalis, to investigate interspecies scaling, and extracts prepared from fertilized eggs at different stages of embryogenesis to study developmental scaling. We seek to provide new insight into the underlying principles of spindle assembly and biological size control, as well as the molecular basis of variation that contributes to genomic instability and evolution.
About the Harland Lab - The objective of our work is to understand early vertebrate development at the molecular level. We study this problem in both the amphibian Xenopus, and in the mouse. Xenopus embryos are large and easily manipulated, so that the function of various macromolecules, such as RNA and protein, can be assayed by microinjection into living embryos. Functional assays in Xenopus can then be complemented by genetic knockouts in the mouse, to gain fuller understanding of the normal requirements for gene action in the developing embryo.
Fish Facility - The Fish Facility, a zebrafish colony, is an important university resource shared by multiple investigators. The Facility is responsible for overseeing the raising of zebrafish at all developmental stages (egg to reproductive adult). The Lab Assistant's primary responsibility will be feeding the fish and ensuring general upkeep and maintenance of the facility, including performing specialized tasks and repairs.
The Aquatic Animals Lab Assistant will support the research of the laboratory by caring the aquatic animals (fish and frogs) in the Heald, Harland and Fish Facility Labs. For 3-4 days per week, the employee is responsible for raising the aquatics animals at all developmental stages (egg to reproductive adult) and for general upkeep and maintenance of the facilities, including specialized tasks and repairs. In addition, the employee will train and supervise work-study students, as well as other lab members on how to perform general lab tasks.
Responsibilities Include: •Prepare food and feed animals. Tanks contain a variable number of animals and the amount of food fed to each tank is calculated by employee based on the tank occupancy. •Wash tanks, sterilize net and glassware. •Routine maintenance of aquaria and recirculating water system. •Record keeping – paper. The daily SOP checklist must be filled out and data as well as any anomalies, recorded. Conductivity and pH values, feeding times, etc. are all recorded on paper. Employee must calculate daily amount of salts and bicarbonate to add to waiter to achieve appropriate conductivity, hardness/alkalinity, and pH and these values are recorded. •Miscellaneous husbandry or record keeping tasks as assigned by senior staff/PI •Analyze animal growth, assess when stocks can be moved from nursey to main facility, and responsibility for moving stocks as appropriate. •Perform specialized system maintenance (Clean and calibrate conductivity and pH probes; disassemble UV sterilization system and perform repairs and/or replace UV bulbs; undertake major system repairs (e.g. pump replacement, leak repair). •Train and supervise work-study students during their shifts and train and/or assist lab members on facility tasks as requested.Knowledge/Skills: •Must be extremely reliable and responsible. •Ability to follow directions, pay attention to detail, and keep careful records. •Once trained, must be able to work independently to perform required tasks and to recognize and report operational problems, and often undertake required repairs. •Ability to perform the physical requirements of the job. •Enjoy working with animals. •Available 3 – 4 days per week, including at least one weekend day. •Must be able to work occasional holidays. Education/Training: •High school diploma and related coursework. This is 50% part-time (20 hours per week) position with full UC benefits.
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